Imaging Proteins, Cells, and Tissues Dynamics during Embryogenesis with Two-Photon Light-Sheet Microscopy

1726-Pos Board B618 Imaging Proteins, Cells, and Tissues Dynamics during Embryogenesis with Two-Photon Light-Sheet Microscopy Thai V. Truong, Daniel B. Holland, Vikas Trivedi, Scott E. Fraser. California Institute of Technology, Pasadena, CA, USA. Two-photon light sheet microscopy combines nonlinear excitation with the novel sheet-illumination, orthogonal to the detection direction, to achieve high penetration depth, high acquisition speed, and low photodamage, compared with conventional imaging techniques. These advantages allow unprecedented observation of the processes that govern embryogenesis, where the ability to image fast the dynamic three dimensional structure of the developing embryo, over extended periods of time, is critical. We present a selection of applications where two-photon light sheet microscopy is utilized to observe the dynamics of proteins, cells, and tissues, toward an understanding of the construction program of the developing embryos.